How to fix this iisue

conda create -n assembly nanoplot flye bandage bwa samtools pilon -c conda-forge -c defaults
Channels:

  • conda-forge
  • defaults
  • biconda
    Platform: linux-64
    Collecting package metadata (repodata.json): failed

UnavailableInvalidChannel: HTTP 404 NOT FOUND for channel biconda https://conda.anaconda.org/biconda

The channel is not accessible or is invalid.

You will need to adjust your conda configuration to proceed.
Use conda config --show channels to view your configuration’s current state,
and use conda config --show-sources to view config file locations.

the channel biconda does not exist on anaconda.org - which is why you are getting the error. Perhaps you meant bioconda?