Illegal Instruction creating Kallisto reference index

Technical Topics Data Science, ML & AI
1

Hi everyone! I am new to data sciences. I need to train to analyze scRNAseq data by myself. To do so, I need to first create an index using reference human genome (dowloaded on Ensembl as FASTA file: Homo_sapiens.GRCh38.cdna.all.fa ). I follow online lectures and he recommands us to use kallisto (0.50.0) in conda environment. I got the line of code to create this index which is: kallisto index -i Homo_sapiens.GRCh38.cdna.all.index Homo_sapiens.GRCh38.cdna.all.fa but after any try I got the error message “Illegal instruction”. I can not find any similar topic so I looking for some help with this.

Thank you

2

Having the exact same issue right now, what CPU are you using?, are you running from native linux or in Windows Subsystem for Linux? I have tried both options on a Ryzen 5 5500 I do not think my CPU is incompatible with kallisto

3

I have fixed the issue by reinstalling it manually.

you can follow the steps here: Source

Good luck!